Journal: NPJ Microgravity

Article Title: BMP9 attenuates microgravity-related disuse osteoporosis by modulating TGFβ and BMP signaling

doi: 10.1038/s41526-025-00510-y

Figure Lengend Snippet: A Alkaline phosphatase staining. B Quantification of alkaline phosphatase enzyme activity. C Osteogenic gene expression following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1 ( n = 3). D Phosphorylation of Smads in marrow stromal cells following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1, as detected by western blot. E BMP-pSmad1/5/8 signaling target gene expression in marrow stromal cells following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1 ( n = 3). ns no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: For chondrogenic differentiation, after infection with Adv-BMP9 or Adv-EGFP, TGFβ3 (10 ng/mL, Topscience, China) was added to F12/DMEM 1:1 (Gibco, USA) containing 10% fetal bovine serum (Shanghai Sangon, China) and 1% penicillin-streptomycin (Solarbio, China).

Techniques: Staining, Activity Assay, Gene Expression, Infection, Control, Virus, Phospho-proteomics, Western Blot, Targeted Gene Expression

Journal: NPJ Microgravity

Article Title: BMP9 attenuates microgravity-related disuse osteoporosis by modulating TGFβ and BMP signaling

doi: 10.1038/s41526-025-00510-y

Figure Lengend Snippet: A Phosphorylation of Smads in primary osteocytes following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1, as detected by western blot. B BMP-pSmad1/5/8 signaling target gene expression in primary osteocytes following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1 ( n = 3). C Intracellular pH changes in primary osteocytes following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1, normalized to control group. D Expression of osteoclastic genes in primary osteocytes following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1 ( n = 3). ns no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: For chondrogenic differentiation, after infection with Adv-BMP9 or Adv-EGFP, TGFβ3 (10 ng/mL, Topscience, China) was added to F12/DMEM 1:1 (Gibco, USA) containing 10% fetal bovine serum (Shanghai Sangon, China) and 1% penicillin-streptomycin (Solarbio, China).

Techniques: Phospho-proteomics, Infection, Control, Virus, Western Blot, Targeted Gene Expression, Expressing

Journal: Comprehensive Physiology

Article Title: Disrupting BMP / TGF ‐β Signaling: Modulation of AQP1 and TGFB1 in Human Pulmonary Microvascular Endothelial Cells

doi: 10.1002/cph4.70066

Figure Lengend Snippet: Effects of silencing the AQP1 gene and exogenous administration of BMP9 on AQP1 and BMP/TGF‐β signaling molecules in human pulmonary microvascular endothelial cells. HPMECs were silenced for the AQP1 gene and the relative mRNA and protein expression of AQP1 (A, B), BMPR2 (C, D), TGFBR1 (E, F), and TGFB1 (G, H) were estimated before and after the exogenous administration of BMP9. Relative mRNA expression is shown in dot plots (dots, individual values; line in the middle, median values; lower and upper lines, 25th and 75th percentiles) (A, n = 6; C, n = 7; E, n = 7; G, n = 6). Protein expression was analyzed by SDS‐PAGE and immunoblotting, and relative expression was estimated by densitometry using β‐tubulin as a loading control. Relative protein expression is shown with bar plots (mean ± SEM; dots, individual values) (B, n = 4; D, n = 4; F, n = 4; H, n = 4). Representative expression of AQP1 (B), BMPR2 (D), TGFBR1 (F), and TGFB1 (H) (upper panels) and β‐tubulin (lower panels) proteins in the AQP1 ‐silenced HPMEC homogenates. Specificity (siRNA negative control), efficiency ( AQP1 siRNA), and the effect of BMP9 exogenous administration on the non‐transfected controls were tested each time to ensure the consistency and reproducibility across the independent experiments. Statistical analysis was performed using the Mann–Whitney test. *, p < 0.05; **, p < 0.01 compared to the non‐transfected control HPMECs.

Article Snippet: HPMECs were treated with BMP9 (5 ng/mL) (OriGene, Rockville, MD, USA) 24 h post‐transfection.

Techniques: Expressing, SDS Page, Western Blot, Control, Negative Control, Transfection, MANN-WHITNEY

Journal: Comprehensive Physiology

Article Title: Disrupting BMP / TGF ‐β Signaling: Modulation of AQP1 and TGFB1 in Human Pulmonary Microvascular Endothelial Cells

doi: 10.1002/cph4.70066

Figure Lengend Snippet: Effects of silencing the TGFB1 gene and exogenous administration of BMP9 on BMP/TGF‐β signaling molecules and AQP1 in human pulmonary microvascular endothelial cells. HPMECs were silenced for the TGFB1 gene and the relative mRNA and protein expression of TGFB1 (A, B), TGFBR1 (C, D), AQP1 (E, F), and BMPR2 (G, H) were estimated before and after the exogenous administration of BMP9. Relative mRNA expression is shown in dot plots (dots, individual values; line in the middle, median values; lower and upper lines, 25th and 75th percentiles) (A, n = 5; C, n = 6; E, n = 4; G, n = 7). Protein expression was analyzed by SDS‐PAGE and immunoblotting, and relative expression was estimated by densitometry using β‐tubulin or Actin as a loading control. Relative protein expression is shown with bar plots (mean ± SEM; dots, individual values) (B, n = 4; D, n = 7; F, n = 4; H, n = 6). Representative expression of TGFB1 (B), TGFBR1 (D), AQP1 (F), and BMPR2 (H) (upper panels) and β‐tubulin (lower panels) proteins in the TGFB1 ‐silenced HPMEC homogenates. Specificity (siRNA negative control), efficiency ( TGFB1 siRNA), and the effect of BMP9 exogenous administration on the non‐transfected controls were tested each time to ensure consistency and reproducibility across the independent experiments. Statistical analysis was performed using the Mann–Whitney test. *, p < 0.05; **, p < 0.01 compared to the non‐transfected control HPMECs.

Article Snippet: HPMECs were treated with BMP9 (5 ng/mL) (OriGene, Rockville, MD, USA) 24 h post‐transfection.

Techniques: Expressing, SDS Page, Western Blot, Control, Negative Control, Transfection, MANN-WHITNEY

Journal: Comprehensive Physiology

Article Title: Disrupting BMP / TGF ‐β Signaling: Modulation of AQP1 and TGFB1 in Human Pulmonary Microvascular Endothelial Cells

doi: 10.1002/cph4.70066

Figure Lengend Snippet: Effects of silencing the TGFB1 gene and exogenous administration of BMP9 on BMP/TGF‐β ligands in human pulmonary microvascular endothelial cells. HPMECs were silenced for the TGFB1 gene, and the relative mRNA and protein expression of BMP9 (A, B) and BMP10 (C, D) were estimated. BMP10 expression was estimated before and after the exogenous administration of BMP9. Relative mRNA expression is shown in dot plots (dots, individual values; line in the middle, median values; lower and upper lines, 25th and 75th percentiles) (A, n = 6; C, n = 5). Protein expression was analyzed by SDS‐PAGE and immunoblotting, and relative expression was estimated by densitometry using β‐tubulin or Actin as a loading control. Relative protein expression is shown with bar plots (mean ± SEM; dots, individual values) (B, n = 4; D, n = 5). Representative expression of BMP9 (B), and BMP10 (D) (upper panels) and β‐tubulin/Actin (lower panels) proteins in the TGFB1 ‐silenced HPMEC homogenates. Specificity (siRNA negative control), efficiency ( TGFB1 siRNA), and the effect of BMP9 exogenous administration on the non‐transfected controls were tested each time to ensure consistency and reproducibility across the independent experiments. Statistical analysis was performed using the Mann–Whitney test. *, p < 0.05 compared to the non‐transfected control HPMECs.

Article Snippet: HPMECs were treated with BMP9 (5 ng/mL) (OriGene, Rockville, MD, USA) 24 h post‐transfection.

Techniques: Expressing, SDS Page, Western Blot, Control, Negative Control, Transfection, MANN-WHITNEY

Journal: EMBO Molecular Medicine

Article Title: BMP-9 mediates fibroproliferation in fibrodysplasia ossificans progressiva through TGF-β signaling

doi: 10.1038/s44321-024-00174-3

Figure Lengend Snippet: Reagents and tools table

Article Snippet: The concentration of BMP-9 in mouse serum was determined using a GDF2/BMP-9 ELISA Kit (LifeSpan BioSciences, LS-F3779) in accordance with the manufacturer’s instructions.

Techniques: Transgenic Assay, Recombinant, Cell Counting, Cell Cycle Assay, Binding Assay, Blocking Assay, Enzyme-linked Immunosorbent Assay, Luciferase, Reporter Assay, Western Blot, Reverse Transcription, Control, Software, Real-time Polymerase Chain Reaction